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Units of activity/mg protein | = | ΔOD340/min6.23 × mg HLADH/mL of assay volume |
Units/mg protein | = | ΔOD340min/20.75 |
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a The optical purities and/or enantiomeric excesses were determined by 1H NMR to be >97%;5 2 was obtained with 85% e.e. |
Appendix
Compounds Referenced (Chemical Abstracts Registry Number)
nicotinamide adenine dinucleotide coenzyme
ethanol (64-17-5)
hydrochloric acid (7647-01-0)
ether (60-29-7)
sodium hydroxide (1310-73-2)
chloroform (67-66-3)
Glycine (513-29-1)
magnesium sulfate (7487-88-9)
lithium aluminum hydride (16853-85-3)
silver carbonate (534-16-7)
cis-1,2-bis(hydroxymethyl) cyclohexane, cis-1,2-Bis(hydroxymethyl)cyclohexane (5059-76-7)
( + )-(1R,6S)-8-OXABICYCLO[4.3.0]NONAN-7-ONE
(1R,2R)-trans-2-hydroxymethylcyclohexanecarboxylic acid
(1R,2R)-trans-1,2-bis(hydroxymethyl)cyclohexane
1(3H)-Isobenzofuranone, hexahydro-, (3aS-cis)- (65376-02-5)